Possibility of lifetime measurement in a flowing medium with phase fluorometry
نویسندگان
چکیده
منابع مشابه
Resolvability of fluorescence lifetime distributions using phase fluorometry.
The analysis of the fluorescence decay using discrete exponential components assumes that a small number of species is present. In the absence of a definite kinetic model or when a large number of species is present, the exponential analysis underestimates the uncertainty of the recovered lifetime values. A different approach to determine the lifetime of a population of molecules is the use of ...
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A new gated form of phase fluorometry for measuring lifetimes is presented. The technique uses a square-wave excitation and gates the detector on only during the off period of the excitation. Using a long-lived sample, this eliminates or reduces errors from scattered light and short-lived fluorescences. Using a square-wave modulated excitation source with a 50% duty cycle, traditional data trea...
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A Microfluidic pH Measurement Device with a Flowing Liquid Junction
The pH values of aqueous solutions are conventionally measured with pH-sensitive electrodes such as glass electrodes or ion-sensitive field-effect transistors (ISFETs) used in conjunction with Ag/AgCl reference electrodes and KCl solutions. The speed of pH measurement with these systems can be deficient, however, as the glass electrode responds slowly during measurements of sample solutions wit...
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ژورنال
عنوان ژورنال: Applied Optics
سال: 1991
ISSN: 0003-6935,1539-4522
DOI: 10.1364/ao.30.001688